Wednesday, 11 May 2011

Chemistry that glows!

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that uses high pressures to force mixtures through the column – hence speeding up the chromatography process. Students simulated HPLC using air from a wash bottle to force the mixture through a glass column. 




Students separated a mixture of polar hydrogen peroxide and sodium salicylate from the organic compound found in glow stick liquid. 






When the glow stick liquid is without hydrogen peroxide it stops glowing. After the separation using HPLC the students’ added hydrogen peroxide back to the organic compound and it started to glow again.

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